Exotic mushrooms, such as Agaricus blazei Murill, Maitake, and Lion's Mane, need to be grown in a sterile environment to prevent contamination by other fungi, bacteria, or pathogens that could compromise their growth. Sterility ensures that the specific spores intended for cultivation dominate the substrate, leading to higher yields and better-quality mushrooms.

Without a sterile environment, unwanted microorganisms can compete with or even destroy the mushroom crop, leading to significant losses. Additionally, maintaining sterility is crucial for growing medicinal mushrooms, as any contamination could impact the safety and efficacy of the final product.

Mushroom spores must not be extracted from the grow house to prevent environmental contamination. The air handling system should be designed to eliminate spore viability, ensuring they don't spread, protecting local ecosystems, and maintaining a controlled growing environment.